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a1M recombinant protein product blog

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Posted on 2014-04-12 11:18:23 by mybiosource_staff
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Tags: Recombinant Protein; A1M; Alpha-1-Microglobulin/Bikunin Precursor (a1M); a1M recombinant protein;
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The a1M ambp (Catalog #MBS2011351) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Alpha-1-Microglobulin/Bikunin Precursor (a1M) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the a1M ambp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The a1M ambp product has the following accession number(s) (GI #6978497) (NCBI Accession #NP_037033.1) (Uniprot Accession #Q64240). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -D NVPTLPDIQV QENFNEARIY GKWFNLAVGS TCPWLRRIKN KMSVSTLVLQ EGATEAEISV TSTQWRKGVC EEISGVYQKT DIDGKFLYHK SKWNATLESY VVHTNYDEYA IFLTKKFSHR HGPTITAKLY GREPQLRDSL LQEFREVALS VGIPENSIVF MADRGECVPG DREVESTSFA RA.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Alpha-1-Microglobulin/Bikunin Precursor (a1M) with the following immunoassay(s):
SDS-PAGE
SDS-PAGE a1M.

Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Alpha-1-Microglobulin/Bikunin Precursor (a1M) are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

Organism: Rattus norvegicus (Rat)
Expression System: Prokaryotic expression
Residues: Asp20~Ala202 (Accession # Q64240) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location: Secreted. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point: 6.1
Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. The following patways have been known to be associated with this gene. Brain Diseases, Disease Models, Animal, Drug-Induced Liver Injury, Hemorrhage, Hypertension, Inflammation, Kidney Diseases, Liver Diseases, Necrosis, Neoplasms are some of the diseases may be linked to Recombinant Alpha-1-Microglobulin/Bikunin Precursor (a1M). Liver, Spleen tissues are correlated with this protein.
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