ADAM17 recombinant protein product blog
Tags: ADAM17; Recombinant Protein; ADAM17 recombinant protein; TACE (TNF-alpha converting enzyme);
The ADAM17 adam17 (Catalog #MBS845409) is an Active Protein produced from Insect cell and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s TACE (TNF-alpha converting enzyme) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC.Active TACE is useful in studying enzyme regulation, determining target substrates, screening TACE inhibitors, or as a positive control in TACE activity assays. We recommend using 0.1-0.2 ?g protein/assay for analyzing TACE activity. Researchers should empirically determine the suitability of the ADAM17 adam17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADAM17 adam17 product has the following accession number(s) (GI #73747889) (NCBI Accession #NP_003174.3) (Uniprot Accession #P78536). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the TACE (TNF-alpha converting enzyme), human recombinant with the following immunoassay(s):
TACE Activity Assay (The activity of TACE was determined using the 20uM substrate Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2. (GM6001 is an inhibitor of TACE. GM6001 at 2uM completely inhibited TACE activity))
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including TACE (TNF-alpha converting enzyme) are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility/Reconstitution Instructions: With sterile deionized water at 0.1ug protein/ul.
Activity (Specifications/test method): >= 280 units/mg
Biological Activity: Activity of TACE was tested using the synthetic peptide, Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2, as a substrate.
Unit Definition: One unit of the recombinant TACE is defined as the amount of enzyme that completely digests 1 nmol of substrate Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa(Dnp)-NH2 per minute at 37 degree C in a reaction solution containing 50 mM Tris, pH 7.5 and 0.1% NP-40.
Handling: Centrifuge the vial prior to opening. Bone Marrow, Brain, Embryonic Tissue, Eye, Kidney, Liver, Lung, Muscle, Ovary, Prostate tissues are correlated with this protein. ADAM17 also interacts with the following gene(s): EGFR, ERBB2, ERBB4, NCSTN, NOTCH1, NOTCH3, PSEN2, TIMP3. Atherosclerosis, Breast Neoplasms, Carcinoma, Hepatocellular, Disease Models, Animal, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Necrosis, Neoplasm Invasiveness are some of the diseases may be linked to TACE (TNF-alpha converting enzyme), human recombinant.