|The CD152 n/a (Catalog #MBS666790) is a Protein produced from BHK cells and is intended for research purposes only. The product is available for immediate purchase. The CD152(CTLA-4)-muIg-FITC reacts with Human, Primate, Mouse, Pig, Cow, Cat and may cross-react with other species as described in the data sheet. MyBioSource\'s CD152 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA).
1:20 (or use 4 mul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD152 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given protein. We\'ve tested the CD152(CTLA-4)-muIg-FITC with the following immunoassay(s):
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including CD152 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Molecular Structure: A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc
Transfectant Cell Line: BHK
Performance: Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 mul of CD152 Ig/FITC at a 1:20 dilution factor. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml CTLA-4 Ig Fusion Protein. Conjugation: FITC
Production: Human CD152 Ig fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. The fusion protein/FITC conjugate is at 20 mug/ml with a Fluorescein/IgG molar ratio of 10.8.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).