|The CD273 n/a (Catalog #MBS666852) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The CD273-muIg-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD273 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the CD273 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:
(221)gtepr gptikpcppc kcpapnllgg psvfifppki kdvlmislsp ivtcvvvdvs eddpdvqisw fvnnvevhta qtqthredyn stlrvvsalp iqhqdwmsgk efkckvnnkd lpapiertis kpgsvrapqv yvlpppeeem tkkqvtvtcm vtdfmpediy vewtnngkte lnykntepvl dsdgsyfmys klgvekknwv ernsyscsvv heglhnhhtt ksfsrtpg(4 55)
Predic ted monomeric (non glycosylat ed) molecular weight: 49 kd. The molecule is dimeric and runs at ~135 kd in SDS-PAGE under native conditions, and ~74 kd reduced
(20)lftvt vpkelyiieh gsnvtlecnf dtgshvnlga itaslqkven dtsphrerat lleeqlplgk asfhipqvqv rdegqyqcii iygvawdyky ltlkvkasyr kinthilkvp etdeveltcq atyplaevsw pnvsvpants hsrtpeglyq vtsvlrlkpp pgrnfscvfw nthvreltla sidlqsqmep rthpt
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given protein. We\'ve tested the CD273-muIg-Biotin with the following immunoassay(s):
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including CD273 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Transfectant Cell Line: CHO
Performance: CD273-muIg/Biotin is functional in EIA as a detection reagent binding to plate-coated recombinant CD279(PD-1). Streptavidin/HRP was used as a secondary detector and TMB/H2 O2 as a chromagen/substrate. Conjugation: Biotin
Production: Human CD274-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).