COL3a1 recombinant protein product blog
Tags: COL3A1; Recombinant Protein; COL3A1 recombinant protein; Collagen Type III Alpha 1 (COL3a1);
The COL3a1 col3a1 (Catalog #MBS2018584) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Collagen Type III Alpha 1 (COL3a1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the COL3a1 col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The COL3a1 col3a1 product has the following accession number(s) (GI #226423933) (NCBI Accession #NP_034060.2) (Uniprot Accession #P08121). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
GKNGAKG EPGARGERGE AGSPGIPGPK GEDGKDGSPG EPGANGLPGA AGERGPSGFR GPAGPNGIPG EKGPPGERGG PGPAGPRGVA GEPGRDGTPG GPGIRGMPGS PGGPGNDGKP GPPGSQGESG RPGPPGPSGP RGQPGVMGFP GPKGNDGAPG KNGERGGPGG PGLPGPAGKN GETGPQGPPG PTGPAGDKGD SGPPGPQGLQ GIP.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Collagen Type III Alpha 1 (COL3a1) with the following immunoassay(s):
SDS-PAGE
Organism Species: Mus musculus (Mouse)
Source: Prokaryotic expression
Residues: Gly434~Pro643
Tags: N-terminal His Tag
Subcellular Location: Secreted. Traits: Freeze-dried powder
Predicted isoelectric point: 9.1
Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage: Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex. Artery, Bone, Connective Tissue, Heart, Joint, Kidney, Lung, Muscle, Other, Skin tissues are correlated with this protein. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Disease Models, Animal, Ehlers-Danlos Syndrome, Fibrosis, Heart Diseases, Hypertension, Hypertrophy, Inflammation, Necrosis, Neoplasms are some of the diseases may be linked to Recombinant Collagen Type III Alpha 1 (COL3a1).