DEFa3 recombinant protein product blog
Tags: Recombinant Protein; DEFA3; DEFA3 recombinant protein; Defensin Alpha 3, Neutrophil Specific (DEFa3);
The DEFa3 defa3 (Catalog #MBS2010173) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Defensin Alpha 3, Neutrophil Specific (DEFa3) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, Reporter Assays, Purification, Amine Reactive Labeling. Researchers should empirically determine the suitability of the DEFa3 defa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The DEFa3 defa3 product has the following accession number(s) (GI #4885179) (NCBI Accession #NP_005208.1) (Uniprot Accession #P59666). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-E PLQARADEVA AAPEQIAADI PEVVVSLAWD ESLAPKHPGS RKNMDCYCRI PACIAGERRY GTCIYQGRLW AFCC.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3) with the following immunoassay(s):
Sequence Information
SDS-Page
Testing Data
Organism: Homo sapiens (Human)
Expression System: Prokaryotic expression
Residues: Pro21~Cys94
Homology: Mouse 34%, rat 33%
Tissue Specificity: Lung, spleen, pancreas.
Subcellular Location: Secreted.
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA,1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 9.7kDa
Accurate Molecular Mass: 11kDa as determined by SDS-PAGE reducing conditions. Blood tissues are correlated with this protein. The following patways have been known to be associated with this gene. DEFa3 also interacts with the following gene(s): CAMP, DEFA1. Blood Coagulation Disorders, Inflammation, Intestinal Diseases, Necrosis are some of the diseases may be linked to Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3).