|The DEFB1 defb1 (Catalog #MBS651077) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The BD1, Recombinant, Human (beta Defensin-1) reacts with Human and may cross-react with other species as described in the data sheet.
The DEFB1 defb1 product has the following accession number(s) (GI #4885181) (NCBI Accession #NP_005209.1) (Uniprot Accession #P60022). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Human defensin-1 (hBD-1; 36 aa): DHYNCVSSGG QCLYSACPIF TKIQGTCYRG KAKCCK.
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Biological Activity: The HBD-1 recombinant protein activity was determined by its ability to chemoattract human peripheral blood dendritic cells using a concentration range of 100-1000ng/ml
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.
Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes. HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract. HD1-3/HNP1-3 make up about 30% of the neutrophil\'s total granule protein. The HNPs are 29 to 30 amino acids long and are identical in sequence except at the N-terminal amino acid. DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively. DEFA2 is identical to both except that it has 29 instead of 30 amino acids.
Endotoxin Level: < 0.1ng/ug (1.0EU/ug). Adenocarcinoma, Carcinoma, Carcinoma, Squamous Cell, Fibrosis, Inflammation, Kidney Diseases, Lung Diseases, Mouth Neoplasms, Neoplasms, Skin Diseases are some of the diseases may be linked to BD1, Recombinant, Human (beta Defensin-1). The following patways have been known to be associated with this gene.