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GCLM recombinant protein product blog

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Posted on 2017-03-07 23:27:04 by mybiosource_staff
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Tags: Recombinant Protein; GCLM; GCLM recombinant protein; Glutamate Cysteine Ligase;
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The GCLM gsh1 (Catalog #MBS2029337) is a Recombinant Protein produced from Host: E Coli
Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Glutamate Cysteine Ligase can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GCLM gsh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GCLM gsh1 product has the following accession number(s) (GI #15236390) (NCBI Accession #NP_194041.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- S EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIQAH EWVPLWLLRY S.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Glutamate Cysteine Ligase, Modifier Subunit (GCLM) with the following immunoassay(s):
SDS-Page
SDS-Page GCLM.

Predicted Molecular Mass: 27.7kDa
Accurate Molecular Mass (KD): 28kDa
Endotoxin: <1.0EU per 1ug (determined by the LAL method). Expression System: Prokaryotic expression
Tag: two N-terminal Tags, His-tag and T7-tag
Organism Species: Homo sapiens (Human)
Fragment: Ser40~Ser251
Usage: Reconstitute in sterile PBS, pH7.2-pH7.4. GCLM also interacts with the following gene(s): GSH2. The following patways have been known to be associated with this gene. Flower, Leaf, Other, Root, Whole Plant tissues are correlated with this protein.
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