|The Granulocyte Colony Stimulating Factor n/a (Catalog #MBS651050) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase.
The Granulocyte Colony Stimulating Factor n/a product has the following accession number(s) (GI #194303452). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Le u-Gly-Pro.
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Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Granulocyte Colony Stimulating Factor are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Biological Activity: G-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is less then 0.1 ng/ml, corresponding to a Specific Activity of 1.27 x 10e8 IU/mg. Protein Content: G-CSF protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Granulocyte Colony Stimulating Factor as a Reference Standard. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Granulocyte Colony Stimulating Factor also interacts with the following gene(s): FLNA.