|The Human HTRA1 htra1 (Catalog #MBS2531908) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531908 CLIA Kit recognizes Human (General) HTRA1.
The HTRA1 htra1 product has the following accession number(s) (GI #225000652) (NCBI Accession #AAI72536.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human HTRA1 (HtrA Serine Peptidase 1) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of HTRA1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HTRA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HTRA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HTRA1. You can calculate the concentration of HTRA1 in the samples by comparing the RLU value of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 78.125-5000pg/mL
Sensitivity: 46.875pg/mL. Blood, Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Eye, Nerve, Placenta, Vascular tissues are correlated with this protein. HTRA1 also interacts with the following gene(s): ARMS2, EGFR, TSC2, XIAP.