MASP2 active protein product blog
Tags: MASP2; Mannose Associated Serine Protease 2 (MASP2); Active Protein; MASP2 active protein;
The MASP2 masp2 (Catalog #MBS2086083) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Mannose Associated Serine Protease 2 (MASP2) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. MyBioSource\'s Mannose Associated Serine Protease 2 (MASP2) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays; In vivo assays. Researchers should empirically determine the suitability of the MASP2 masp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The MASP2 masp2 product has the following accession number(s) (GI #21264363) (NCBI Accession #NP_006601.2) (Uniprot Accession #O00187). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the Active Mannose Associated Serine Protease 2 (MASP2) with the following immunoassay(s):
Sequence
Activity Data (MASP2 (Mannan-binding lectin serine protease 2) is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. The preproprotein of MASP2 is proteolytically processed to generate An and B chains that heterodimerize to form the mature protease, which is able to associate with MBL2. Thus, a functional binding ELISA assay was constructed to detect the association of rhMASP2 with MBL2. Briefly, rhMASP-2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MBL2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MASP-2 pAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated for 15-25 minutes at 37oC. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MASP2 with MBL2 was shown in Figure 1 and this effect was in a dose dependent manner.)
Gene Sequencing (extract)
SDS-Page
Western Blot (WB) (Sample: Recombinant MASP2, Human;
Antibody: Rabbit Anti-Human MASP2 Ab (MBS2004712))
Source: Prokaryotic expression.
Residues: Ile445~Ile683
Tags: N-terminal His-tag. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point: 5.7
Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. The following patways have been known to be associated with this gene. Brain Diseases, Carcinoma, Hepatocellular, Disease Models, Animal, Fibrosis, Hepatitis, Inflammation, Kidney Diseases, Liver Diseases, MASP2 Deficiency, Nephritis are some of the diseases may be linked to Active Mannose Associated Serine Protease 2 (MASP2). Brain, Embryonic Tissue, Liver, Lung, Placenta, Thyroid tissues are correlated with this protein. MASP2 also interacts with the following gene(s): FCN2, MBL2, SERPING1.