NAGase recombinant protein product blog
Tags: NAGase; Recombinant Protein; N-Acetyl Beta-D-Glucosaminidase (NAGase); NAGase recombinant protein;
The NAGase mgea5 (Catalog #MBS2012338) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. MyBioSource\'s N-Acetyl Beta-D-Glucosaminidase (NAGase) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).(May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the NAGase mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NAGase mgea5 product has the following accession number(s) (GI #215490056) (NCBI Accession #NP_001135906.1) (Uniprot Accession #O60502). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase) with the following immunoassay(s):
Sequence Information
Gene Sequencing (extract)
SDS-Page
Source: Prokaryotic expression
Residues: Ser6~Lys240
Tags: N-terminal His Tag
Subcellular Location: Nucleus, Cytoplasm, Chromosome. Traits: Freeze-dried powder
Predicted isoelectric point: 5.2
Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Cardiovascular Diseases, Inflammation, Kidney Diseases, Necrosis are some of the diseases may be linked to Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase). NAGase also interacts with the following gene(s): OGT. Bladder, Blood, Brain, Embryonic Tissue, Kidney, Lymph, Lymph Node, Muscle, Skin, Vascular tissues are correlated with this protein.