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NCL recombinant protein product blog

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Posted on 2016-05-26 11:39:18 by mybiosource_staff
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Tags: Recombinant Protein; NCL; NCL recombinant protein; Nucleolin (NCL);
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The NCL ncl (Catalog #MBS2010481) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Nucleolin (NCL) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the NCL ncl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NCL ncl product has the following accession number(s) (GI #84875537) (NCBI Accession #NP_035010.3) (Uniprot Accession #P09405). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Nucleolin (NCL) with the following immunoassay(s):
SDS-PAGE
SDS-PAGE NCL.

Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Nucleolin (NCL) are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

Organism: Mus musculus (Mouse)
Expression System: Prokaryotic expression
Residues: Tyr353~Ser568 (Accession # P09405) with N-terminal His-Tag. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. The following patways have been known to be associated with this gene. Atrophy, Brain Diseases, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Liver Diseases, Neoplasms, Nervous System Diseases, Pain, Seizures are some of the diseases may be linked to Recombinant Nucleolin (NCL). Brain, Embryonic Tissue, Eye, Intestine tissues are correlated with this protein.
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