PKM2 recombinant protein product blog
Tags: PKM2; Recombinant Protein; PKM2 recombinant protein; Pyruvate Kinase, Muscle (PKM2);
The PKM2 pkm (Catalog #MBS2010633) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Pyruvate Kinase, Muscle (PKM2) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The PKM2 pkm product has the following accession number(s) (GI #16757994) (NCBI Accession #NP_445749.1) (Uniprot Accession #P11980). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VIC ATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAVF HRLLFEELAR ASSQSTDPLE AMAMGSVEAS YKCLAAALIV LTESGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDAVLD AWAEDVDLRV NLAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Pyruvate Kinase, Muscle (PKM2) with the following immunoassay(s):
SDS-Page
Organism: Rattus norvegicus (Rat)
Expression System: Prokaryotic expression
Residues: Val324~Pro531 (Accession # P11980) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location: Cytoplasm. Nucleus. Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point: 9
Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4. Carcinoma, Cardiovascular Diseases, Cell Transformation, Neoplastic, Fibrosis, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Neoplasms, Nervous System Diseases are some of the diseases may be linked to Recombinant Pyruvate Kinase, Muscle (PKM2). The following patways have been known to be associated with this gene. Brain, Colon, Dorsal Root Ganglion, Embryonic Tissue, Eye, Heart, Kidney, Liver, Lung, Muscle tissues are correlated with this protein.