RAD50 recombinant protein product blog
Tags: Recombinant Protein; Rad50; Rad50 recombinant protein; DNA Repair Protein RAD50 (RAD50);
The RAD50 rad50 (Catalog #MBS2009740) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s DNA Repair Protein RAD50 (RAD50) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; WB.(May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the RAD50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAD50 rad50 product has the following accession number(s) (GI #19924129) (NCBI Accession #NP_005723.2) (Uniprot Accession #Q92878). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DVCGSQDFES DLDRLKEEIE KSSKQRAMLA GATAVYSQFI TQLTDENQSC CPVCQRVFQT EAELQEVISD LQSKLRLAPD KLKSTESELK KKEKRRDEML GLVPMRQSII DLKEKEIPEL RNKLQNVNRD IQRLKNDIEE QETLLGTIMP EEESAKVCLT DVT.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant DNA Repair Protein RAD50 (RAD50) with the following immunoassay(s):
SDS-Page
Organism Species: Homo sapiens (Human)
Source: Prokaryotic expression
Residues: Asp631~Thr793
Tags: N-terminal His Tag
Subcellular Location: Nucleus
Traits: Freeze-dried powder. Predicted isoelectric point: 5.2
Predicted Molecular Mass: 20.2kDa
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. RAD50 also interacts with the following gene(s): ATM, BRCA1, H2AFX, MDC1, MRE11A, NBN, RAD51, SPO11, TERF2, TERF2IP. Anemia, Asthma, Brain Diseases, Breast Neoplasms, Fibrosis, Lung Diseases, Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Ovarian Neoplasms are some of the diseases may be linked to Recombinant DNA Repair Protein RAD50 (RAD50). Blood, Bone, Bone Marrow, Brain, Kidney, Liver, Lung, Prostate, Skin, Testis tissues are correlated with this protein. The following patways have been known to be associated with this gene.