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Radius 24-Well Cell Migration Assay, (ECM Array Coated) assay kit product blog

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Posted on 2017-03-04 01:50:19 by mybiosource_staff
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Tags: Assay Kit; Radius 24-Well Cell Migration Assay, (ECM Array Coated); Radius 24-Well Cell Migration Assay, (ECM Array Coated) assay kit;
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The Radius 24-Well Cell Migration Assay, (ECM Array Coated) n/a (Catalog #MBS169144) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Radius 24-Well Cell Migration Assay, (ECM Array Coated) with the following immunoassay(s):
Testing Data
Testing Data Radius 24-Well Cell Migration Assay, (ECM Array Coated).

Testing Data #2
Testing Data #2 Radius 24-Well Cell Migration Assay, (ECM Array Coated).

Testing Data #3
Testing Data #3 Radius 24-Well Cell Migration Assay, (ECM Array Coated).

Testing Data #4
Testing Data #4 Radius 24-Well Cell Migration Assay, (ECM Array Coated).

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). The Radius Cell Migration Assay Kit utilizes a proprietary 24-well plate, which has been pre-coated with various ECM proteins (see Migration Plate Layout below), to monitor the migratory properties of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot (Radius Gel) where cells cannot attach. When adherent cells are seeded in the Radius Cell Migration well, they attach outside of the Radius Gel coated area. Once firm cell attachment is achieved, the hydrogel is quickly removed to expose a cell-free region to study cell migration/closure. This format provides a robust in vitro system to measure 2-D cell migration, screen potential inhibitors and study cytoskeleton reorganization events. The Radius Cell Migration Assay Kit is designed for High Content Analysis applications or imaging software and is adaptable to liquid handling equipment. Each kit provides sufficient quantities to perform 24 migration tests (18 ECM protein-coated wells, 6 uncoated wells).

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