Snca recombinant protein product blog
Tags: Alpha Synuclein; SNCA; Recombinant Protein; SNCA recombinant protein;
The Snca snca (Catalog #MBS8001847) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Alpha Synuclein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), SDS-PAGE, In vivo assay, In vitro assay. Researchers should empirically determine the suitability of the Snca snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The Snca snca product has the following accession number(s) (GI #109638747) (NCBI Accession #NP_001035916.1) (Uniprot Accession #O55042). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the Alpha Synuclein Protein with the following immunoassay(s):
Testing Data (Active alpha synuclein aggregate (SPR-324) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-323). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-324) is combined with 100 uM of active alpha Synuclein monomer (SPR-323), as compared to active alpha Synuclein aggregate (SPR-324) and active alpha Synuclein monomer (SPR-323) alone. Thioflavin T ex = 450 nm, em = 485 nm.)
SDS-Page (SDS-PAGE of ~14 kDa Active Mouse Recombinant Alpha Synuclein Protein Monomer (SPR-323). Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Monomer (2 ug) (SPR-323).)
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Alpha Synuclein are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Species: Mouse
Target: Alpha Synuclein
Cellular Localization: Cytoplasm; Membrane; Nucleus
Storage Buffer: PBS pH 7.4
Biological Activity: 100 uM alpha synuclein protein monomer seeded with 10 nM alpha synuclein protein aggregate in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated an increased fluorescence intensity after incubation at 37 degree C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader. Tag Information: No tag. Bone, Brain, Embryonic Tissue, Eye, Liver, Lung, Olfactory Mucosa, Prostate, Skin, Sympathetic Ganglion tissues are correlated with this protein.