Tnf recombinant protein product blog
Tags: TNF; Recombinant Protein; TNF recombinant protein; Tumor Necrosis Factor alpha, Rat;
The Tnf tnf (Catalog #MBS650055) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The Tnf tnf product has the following accession number(s) (GI #6981662) (NCBI Accession #NP_036807.1) (Uniprot Accession #P16599). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Ar g-Ser-Ser.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Tumor Necrosis Factor alpha, Rat are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Biological Activity: Rat TNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 5x107 IU/mg.
Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level: 0.1ng/ug (IEU/ug) of Rat TNF-alpha. Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.25 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-an as a Reference Standard.
Solubility: It is recommended to reconstitute the lyophilized Rat TNF-alpha in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The following patways have been known to be associated with this gene. Atherosclerosis, Fibrosis, Inflammation, Insulin Resistance, Liver Diseases, Lung Diseases, Necrosis, Pain, Skin Diseases, Vascular Diseases are some of the diseases may be linked to Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa). Blood, Prostate tissues are correlated with this protein.