|The TOP1 topa (Catalog #MBS2029560) is a Recombinant Protein produced from Host: E Coli
Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Topoisomerase I can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the TOP1 topa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TOP1 topa product has the following accession number(s) (GI #787856383) (NCBI Accession #AKA25541.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA KIKKEKENGF SSPPQIKDEP EDDGYFVPPK EDIKPLKRPR DEDDADYKPK KIKTEDTKKE KKRKLEEEED GKLKKPKNKD KDKKVPEPDN KKKKPKKEEE QKWKWWEEER YPEGIKWKFL EHKGPVFAPP YEPLPENVKF YYDGKVMKLS.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Topoisomerase I (TOP1) with the following immunoassay(s):
Predicted Molecular Mass: 34.0kDa
Accurate Molecular Mass (KD): 40kDa
Endotoxin: <1.0EU per 1ug (determined by the LAL method). Expression System: Prokaryotic expression
Tag: two N-terminal Tags, His-tag and T7-tag
Organism Species: Homo sapiens (Human)
Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.