|The TWEAK n/a (Catalog #MBS537565) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s TWEAK can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the TWEAK n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:
Region of TWEAK protein correspond ing to amino acids MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including TWEAK are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Protein Type: Recombinant
Biological Significance: TWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF kb activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-gamma. TWEAK also promotes proliferation and migration of endothelial cells.
Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml.
Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug). Expression System: E Coli.