VEGF-C152S recombinant protein product blog
Tags: Recombinant Protein; VEGF-C152S; VEGF-C152S recombinant protein;
The VEGF-C152S vegfc (Catalog #MBS692062) is an Active Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The Rat VEGF-C152S reacts with Rat and may cross-react with other species as described in the data sheet.The VEGF-C152S vegfc product has the following accession number(s) (GI #16758992) (NCBI Accession #NP_001165097) (Uniprot Accession #P15692). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DTVKLAAAHY NTEILKSIDN EWRKTQCMPR EVCIDVGKEF GAATNTFFKP PSVSVYRCGG CCNSEGLQCM NTSTGYLSKT LFEITVPLSQ GPKPVTISFA NHTSCRCMSK LDVYRQVHSI IHHHHHH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the Rat VEGF-C152S with the following immunoassay(s):
SDS-PAGE (SDS-PAGE analysis of recombinant rat VEGF-C152 mutant. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.)
Testing Data (In vivo: The lymphangiogenic response to recombinant rat VEGFCC152S loaded in our biopolymeric albumin-alginate microcapsules for targeted slow-release was assayed in male Wistar rats. Briefly, after ischemiareperfusion injury to induce myocardial infarction, VEGF-CC152S at the dose of 1.5 or 5 ug per heart was injected intra-myocardially. Lymphatic responses in the myocardium were analyzed by IHC at 3 weeks post-MI using LYVE1 antibody. Results are expressed as lymphatic vessel to cardiomyocyte ratio (mean + sem).
The experiments were performed by the research group of Prof. Dr. E. Brakenhielm � Rouen University (see also: Henri O et al., Circulation, March 2016, DOI: 10.1161))
Testing Data (In vitro: The proliferative response to recombinant rat VEGF-CC152S was assayed in VEGFR3-expressing porcine aortic endothelial (PAE) cells. Briefly, cells were plated in 12-well plates and incubated in DMEM medium supplemented with 1% fetal calf serum for cell cycle arrest 24h prior to stimulation of cell proliferation with recombinant VEGF-CC152S at the concentrations of 50 and 100 ng/mL. After 48h in culture, the cell proliferative response was assayed (WST-1 colorimetric assay), and the results expressed as % of control non-stimulated cells (mean + sem).)
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including VEGF-C152S are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Buffer: 50 mM acetic acid
Stabilizer: BSA
Reconstitution: Centrifuge the vial prior to opening! The lyophilized VEGF-C152S should be reconstituted in PBS or medium to a concentration not lower than 50 ug/ml.
Biological Activity: (A) The proliferative response to rrVEGF-CC152S was assayed in VEGFR3-expressing porcine aortic endothelial (PAE) cells (in vitro). (B) The lymphangiogenic response to rrVEGF-CC152S loaded in a biopolymeric albumin-alginate microcapsules for targeted slow-release was assayed in male Wistar rats.
Protein RefSeq: NP_446105.1
mRNA RefSeq:: NM_053653.1.